Comparative Study between the CRISPR/Cpf1 (Cas12a) and CRISPR/Cas9 Systems for Multiplex Gene Editing in Maize

Although the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been proved to be an efficient multiplex gene editing in maize, it was still unclear how CRISPR/Cpf1 (Cas12a) would perform for maize. To this end, study compared and CRISPR/Cas9 The bZIP transcription factor Opaque2 (O2) used as target both systems. We found that T0 T1 generations, showed lower efficiency than system. However, T2 generation, generated more types of new mutations. While tended edit within on-target range, preferred between targets. also system, positively correlated with expression level Cpf1. In conclusion, offers alternative choices target-site selection acceptable maize is a valuable choice crops.